qPCR analysis showed a decrease in highly upregulated gene levels associated with HSC activation, namely Acta2, Col1a2, Col3a1, TIMP-1, TGF-b, and PDGFR-b, in the neratinib-treated group compared to that in the vehicle-treated fibrosis group (Fig. 4E). This evidence concerns the gene TIMP1 and fibrosis.