Then, we investigated the chromatin accessibility of the stemness genes in cultured CRC cells under either acidic or alkaline culture condition by analyzing assay transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) signals from upstream of transcription start sites (TSSs) throughout the whole ranges of the SOX2, OCT4, CD44, and NANOG genes. Here, NANOG is linked to colorectal carcinoma.