Remarkably, we also detected loss of ZO-1 in the Tbk1+/− ALS mouse model at P270 (27.0% ± 0.6% versus 22.7% ± 0.7%; Tbk1+/− versus WT; P < 0.01; Fig 2G and H), with a significant increase at P450 (31.4% ± 2.2% versus 23.5% ± 1.6%, respectively, in Tbk1+/− versus WT; P < 0.0001; Tbk1+/− P270 versus Tbk1+/− P450: P < 0.01; Fig 2G and H). The gene discussed is TBK1; the disease is amyotrophic lateral sclerosis.