Therefore, to investigate the effects of TGFβ on PDGFRβ and THBS4 overexpression in CRC, we examined the mRNA levels of PDGFRβ and THBS4 after treatment with TGFβ for 12 h in DLD-1 cells, which is a colon cancer cell line. The gene discussed is TGFB1; the disease is malignant colon neoplasm.