To investigate how THBS4 is stimulated and secreted by colon cancer cells, and whether the colon cancer cells can affect neighboring cells around it, colonic myofibroblast CCD-18co cells were cultured in the conditioned medium (CM) of DLD-1 cells or SW-48 cells stimulated with respective factors (Figure 6A,B). This evidence concerns the gene THBS4 and malignant colon neoplasm.