PIK3CA and acute myeloid leukemia: The major findings of the current study are: (i) WTH and CoA possess potent anti-proliferative and pro-apoptotic activities against AML cell models; (ii) WTH has more potent cytotoxic activity than CoA; (iii) both withanolides modulate the expression of proteins involved in regulating key cellular processes including metabolism, signaling, cell cycle, protein degradation and gene expression; and (iv) both withanolides block PI3K and MAPK oncogenic pathways and increase the phosphorylation of proteins in the caspase cascade leading to apoptosis.