To demonstrate whether TGF-β exerts a protective effect against high glucose concentration-triggered oxidative damage, we knocked down TGF-β in cells and cultured them in 5.5, 25, 50, and 100 mM glucose supplementation for at least 3 weeks, thereby detecting the changes in the proliferation rate of TGF-β knockdown on hyperglycemia-induced oxidative damage. This evidence concerns the gene TGFB1 and Hyperglycemia.