As shown in the example images in Figure 4, B16F10 tumor fragments retained much of their live stains (green in images), and did not gain annexin V-APC staining (red in images) between Days 1 (Figure 4c,e) and 4 (Figure 4d,f), indicating that the viability remained high in the fragments over several days in perfusion flow. The gene discussed is APC; the disease is neoplasm.