Immunohistochemical analyses of tumors confirmed increased protein levels of NLRP1, IL-1β, and Notch1 in tumor cells in mice receiving TMZ treatment compared with those receiving vehicle control (Figure 6C–E), demonstrating that TMZ induced similar biological effects on tumor cell growth, Notch signaling, and NLRP1 inflammasome activation in vivo as observed in 2D cell culture. This evidence concerns the gene NLRP1 and neoplasm.