Encouraged by the rising demand for phenotype-specific and variant-specific screening kits for deafness, we developed a genetic diagnostic kit (U-TOPTM HL Genotyping Kit Ver2), characterized by real-time PCR-based melting array techniques and peptide nucleic acid (PNA) probes, for screening of 11 variants from five deafness genes, which comprised two ANSD genes (OTOF and ATP1A3) and three SNHL genes (MPZL2, COCH, and TMC1), with a distinct auditory phenotype among Koreans. Here, TMC1 is linked to deafness.