A one-way ANOVA revealed a group difference at 0 min, such that exercise resulted in lower plasma insulin levels only in males following long-term HF diet exposure (group F (1,42) = 4.05, p < 0.05; post hoc male Sed vs. WR p < 0.01 and female Sed vs. WR p > 0.13). This evidence concerns the gene INS and hydrops fetalis.