Considering that the vast majority of HSCR patients display aganglionosis over only a short segment of the distal colon [1, 2], such intervention might well be sufficient to prevent megacolon occurrence in many male individuals (up to 50% of them according to our human DDX3Y immunofluorescence data; Fig 2 and Table 1). This evidence concerns the gene DDX3Y and Aganglionic megacolon.