In the nos > RpS13 RNAi testes, we found that Eya‐positive (Eya+) and Zfh1‐positive (Zfh1+) cyst cells accumulated and even Eya/Zfh1 double positive (Eya+/Zfh1+) cells existed, indicating the formation of abnormal characteristics of cyst stem cells or mature cyst cells (Figure 1D‐F). This evidence concerns the gene RPS13 and cyst.