Since many ctDNA reports are based on pre-selected cancer gene panels containing recurrently mutated genes, and since TP53 was the most frequently mutated gene in our cohort, we performed the above analyses using only the 18 TP53 variants measured in plasma, of which 2 were shared by 2 different patients (p.R81X and p.R150W) (Supplementary Table S7). The gene discussed is TP53; the disease is cancer.