The primary aims of our prospective study were: (1) characterize the concentrations of several PIs (ie, ATA, α2MG, α1PI, and α2AP) and proinflammatory cytokines (ie, IL‐2, IL‐6, IL‐8, and TNF‐α) in dogs diagnosed with AP and in healthy control dogs and (2) investigate the associations of these analytes with short‐term death in dogs with AP. This evidence concerns the gene SERPINF2 and alkaline phosphatase measurement.