To determine why infection with Gram-negative bacteria induced the expression of Siglec-E but infection with Gram-positive bacteria reduced the expression of Siglec-E, we tested the effects of TLR4 ligand LPS and TLR2 ligand LTA on Siglec-E expression in neutrophils isolated from wild-type mouse bone marrow (Figure 7H). The gene discussed is TLR4; the disease is infection.