The GSEA of two GEO data sets (GSE62254 and GSE15459) and TCGA gastric cancer data set revealed that GJA1 may be a downstream target gene for SLC52A3 (Figure S2), we further investigated whether SLC52A3 regulated malignant phenotype of gastric cancer cells via regulating GJA1. We firstly detected GJA1 expression in SLC52A3 overexpression gastric cancer cells and found that overexpression of SLC52A3 resulted in decreased GJA1 (Figure 5A). The gene discussed is GJA1; the disease is gastric cancer.