Our analysis revealed two of the most frequent primary mutations shared in LHON, m.11778G>A in MT-ND4 and m.3460G>A in MT-ND1, as well as one mutation m.8836A>G in MT-ATP6 which is consider to be a rare cause of LHON. The gene discussed is MT-ND4; the disease is Leber hereditary optic neuropathy.