IRGM and Huntington disease: In western blot assays (Fig 5A), the expression levels of IRGM1, OSBPL2 and SERPIN B6, which were up-regulated in HD mice and hnRNP H, HTT, SAMM50, UQCRQ and HOMER1, whose expression was instead decreased in HD mice were evaluated on total protein extracts of three WT and three HD mice (the same samples employed for the proteomic experiment), in duplicate.