We performed a correlation analysis of various macrophage markers in esophageal tumor and demonstrated that PU.1 expression strongly correlates with that of CD68 (r = 0.833, p ≤ 0.001), CD163 (r = 0.500, p ≤ 0.001), and CD206 (r = 0.250, p = 0.043) with the strongest correlation observed for CD68 (Figure 2). This evidence concerns the gene SPI1 and neoplasm of esophagus.