Realising the emergence of microRNA as a suitable traditional biomarker in diagnosing various metabolic disorders including its expression in relevance to T2DM, the present study aims to evaluate the role of microRNA-330 and E2F1 mRNA regulation in T2DM cases in Indian population primarily to understand the pathogenesis in newly diagnosed type 2 diabetes mellitus patients. This evidence concerns the gene E2F1 and type 2 diabetes mellitus.