To investigate the tumorigenesis effects of IGF2BP1 and NONO in vivo, we subcutaneously injected wild type (WT), IGF2BP1-knockout (IGF2BP1-cas9), or NONO-knockout (NONO-cas9) MHCC97H cells in nude mice and found that IGF2BP1 knockout or NONO knockout caused less tumor formation and evidently reduced tumor size, compared with control group (Fig. 7a). The gene discussed is IGF2BP1; the disease is neoplasm.