Given that NONO is involved in regulating nuclear retention of mRNAs [37], we further examined the effects of NONO on ACLY mRNA distribution and found that NONO knockdown significantly decreased ACLY mRNA expression both in the cytoplasm and nucleus of DEN stimulated HCC cells (Fig. 5b), which may partially explain why NONO knockdown decreasing IGF2BP1 binding to ACLY mRNA (Fig. 5a). This evidence concerns the gene ACLY and hepatocellular carcinoma.