Furthermore, we found that IGF2BP1 knockdown slightly increased NONO binding to ACLY mRNA in DEN stimulated HCC cells (Fig. 5c), and significantly increased ACLY mRNA expression in the nucleus of DEN stimulated HCC cells, but decreased ACLY mRNA expression in the cytoplasm (Fig. 5d), suggesting that NONO functions on the upstream of nucleocytoplasmic export of ACLY mRNA. The gene discussed is ACLY; the disease is hepatocellular carcinoma.