ACLY and hepatocellular carcinoma: Heatmap analysis showed that NONO binding genes involved FA biosynthesis, including ACLY, short chain enoyl-CoA hydratase 1 (ECHS1), acyl-CoA dehydrogenase very long chain (ACADVL), acyl-CoA dehydrogenase short chain (ACADS), SCD1, FASN and ACC, were significantly upregulated in HCC cells under DEN stimulation, and ACLY mRNA expression was the most upregulated (Fig. 1h).