Heatmap analysis showed that NONO binding genes involved FA biosynthesis, including ACLY, short chain enoyl-CoA hydratase 1 (ECHS1), acyl-CoA dehydrogenase very long chain (ACADVL), acyl-CoA dehydrogenase short chain (ACADS), SCD1, FASN and ACC, were significantly upregulated in HCC cells under DEN stimulation, and ACLY mRNA expression was the most upregulated (Fig. 1h). This evidence concerns the gene ECHS1 and hepatocellular carcinoma.