Plasma insulin levels continued to rise during the study on the HFD and were significantly increased as compared to the chow diet at t = 28 (5.1-fold, p < 0.001), while glucose levels remained similar, resulting in a significantly higher insulin resistance on the HFD (4.8-fold increase in HOMA-IR at t = 28, p = 0.001). This evidence concerns the gene INS and Insulin resistance.