Quantitative RT-PCR and immunoblotting results showed that knocking out lnc-TSI remarkably activated EMT of ccRCC cells, including decreasing E-cadherin and increasing N-cadherin, Vimentin, and Snail in TGF-β1-stimulated ccRCC cells (Figures S2A–S2D; Figure 4A). The gene discussed is VIM; the disease is nonpapillary renal cell carcinoma.