As depicted in Figure 4, DFU subjects with the homozygous mutant TT genotype were observed to have a significant increase in IL-6 and TNF-α expression when compared to T2DM and DFU subjects with homozygous wild CC genotype, whereas IL-10 expression was significantly decreased in DFU subjects with the homozygous mutant TT genotype when compared to T2DM and DFU subjects with homozygous wild CC genotype. This evidence concerns the gene TNF and type 2 diabetes mellitus.