RAF1 and infection: To determine the effect of modulation of PKM2 on DENV 2 protein expression, cells were again infected under an ADE-infection protocol and were then treated with the PKM2 inhibitor or PKM2 activator at 0, 3 and 24 h p.i. At 48 h p.i., proteins were extracted and expression of the structural E protein and the non-structural NS1 and NS5 proteins were determined by western blot analysis.