FGF19 transgenic mice have a significantly reduced fat mass resulted from increased metabolic rate that leads to enhanced energy expenditure, and do not become diabetic or obese when fed a high-fat diet (HFD).530 In HFD fed mice, FGF19 increases the metabolic rate simultaneously with an increased fatty acid oxidation, and alleviates the obesity in ob/ob mice.529 Adeno-associated virus (AAV) delivery of FGF15 and FGF19 reduces fat mass and increases energy expenditure in diet-induced obesity (DIO) mice, and FGF19 can also overt diabetes in db/db mice.531. This evidence concerns the gene FGF19 and diabetes mellitus.