To identify how LINC00301 regulates CD4+Foxp3+ Tregs, we first examined TGF-β1 levels in the culture supernatant of NSCLC cells and normal lung epithelial cells, and the results showed a relative TGF-β1 level (ELISA) in LA-4 and KLN-205 cells than that of in MLE-12 cells (Additional file 1: Fig. S3C), and also LA-4 and KLN-205 cells showed a relatively higher TGF-β1 mRNA level than that of in MLE-12 cells (Additional file 1: Fig. S3D). The gene discussed is CD4; the disease is non-small cell lung carcinoma.