In breast cancer (MCF7 and MDA-MB-231) cell lines, EGCG (20 μM; 48 h) reduced DNMT expression and activity, which in turn decreased the methylation status of SCUBE2 (signal peptide-CUB (complement protein C1r/C1s, Uegf, and Bmp1)-EGF (epidermal growth factor) domain-containing protein 2). This evidence concerns the gene DNMT1 and breast cancer.