We used a potent single guide RNA (sgRNA) we previously found to efficiently edit human CD34+ HSPCs at the hemoglobin beta (HBB) locus and an single-stranded oligodeoxynucleotides (ssODN) donor template designed to modify the causative HBB mutation involved in sickle cell disease (SCD) (Figure S1A; Cradick et al., 2013; DeWitt et al., 2016). The gene discussed is HBB; the disease is sickle cell disease.