Through transfection of siRNAs specific for MAP2K3 and GADD45B, as well as plasmids harboring full-length human MAP2K3 and GADD45B sequences (Supplementary Fig. S7A, B), we found MAP2K3 and GADD45B inhibited esophageal cancer cell proliferation (Supplementary Fig. S7C, D). This evidence concerns the gene MAP2K3 and esophageal cancer.