As SFE induced mitochondrial apoptosis through the activated p53, and several observations implied p53 could inactivate the Wnt pathway in various ways46–48, and we speculated that there was a connection between cell apoptosis caused SFE-treatment and metastasis, which involved p53 and the Wnt pathway in esophageal cancer cells. The gene discussed is TP53; the disease is esophageal cancer.