To determine whether the overexpression of the SOCS proteins can directly affect melanoma invasion and/or suppress the effect of IFNβ, we prepared stable cell lines bearing EGFP-tagged doxycycline-inducible SOCS1 and SOCS3 (iSOCS1 and iSOCS3) gene expression constructs (Figure S6a). Here, SOCS3 is linked to melanoma.