From the seven PDA cell lines, Capan-1 and BxPC-3 were the ones chosen to knockdown α2,3-STs (ST3GAL4 and ST3GAL3) because they presented the highest levels of sLex on their cell surface, in total protein lysates and conditioned media, and also showed high to moderate levels of sLea. This evidence concerns the gene ST3GAL4 and Patent ductus arteriosus.