They obtained 3 variants of SH-SY5Y over expressing tau (0N4R), namely wild type (WT), a variant with single point mutation P301L (which is used in common) and K280q (which is 4-fold gene mutation in the tau protein gene DK280, P301L, V337M, R406W), which was used to enhance tauopathy. The gene discussed is MAPT; the disease is tauopathy.