CpG island hypermethylation in promoter region of TGSs as death-associated protein kinase (DAPK), O6-methylguanine DNA methyltransferase (MGMT) and runt-related transcription factor 3 (RUNX3) have been consistently observed in many human cancers (Kito et al., 2001; Raveh and Kimchi, 2001; Esteller and Herman, 2004; Subramaniam et al., 2009; Asada et al., 2013). This evidence concerns the gene RUNX3 and cancer.