GFAP and Spinocerebellar ataxia type 3: To investigate whether BBB impairment was associated with neuroinflammation and, specifically to astrogliosis and microgliosis, we carried out immunohistochemistry against CoIV (blood vessels marker), GFAP (astrocyte marker) and Iba1 (microglia marker) in striata tissue from MJD patients and control individuals.