Therefore, the present study was designed to: (a) elucidate the BDNF desorption from well-characterized PEG-ylated PAMAM dendrimer nanoparticles, (b) investigate diffusion and cytotoxicity of PEG-ylated BDNF-PAMAM dendrimer electrostatic complex in differentiated neuroblastoma SH-SY5Y cells exposed to 6-hydroxydopamine (6-OHDA) in real-time up to 24 h after administration. Here, BDNF is linked to neuroblastoma.