AKT1 and breast carcinoma: Western blot analysis showed that the PI3K/AKT signaling pathway (assessed by AKT activation, P-AKT immuno-blot) was highly up-regulated in all GB cells studied in comparison with the SC-1800, non-neoplastic astrocyte cell line, and even when compared to the breast carcinoma cell line, MDA-MB-231 (Figure S5).