Gene expression analysis in Low Grade Gliomas (LGG) compared to GB or normal brain tissue showed that the majority of the glycolysis related genes, namely LDHA, PDK1, SLC2A1, and CA9, were expressed at similar or even slightly lower levels in LGG compared to normal brain tissue; whereas PFKFB4 expression increased in a tumor grade dependent manner and PFKFB3 expression was similar in LGG and GB (Figure S6). This evidence concerns the gene PFKFB4 and central nervous system cancer.