Since the Y2H interactome was obtained from SKOV-3 cells, not considered as a cell line representative of the more frequent HGSOC, although extensively used in EOC studies [32], and from tissue extracted from primary transitional cell carcinoma (TCC) of the ovary, which is a relatively unfrequently diagnosed serous EOC, we decided to validate HMGB2 interactions with MIEN1 and NOP53 by co-immunoprecipitation, an orthogonal method to the Y2H approach, and using two EOC cell lines, SKOV-3 and PEO1 representatives of ENOC/CCOC and HGSOC respectively [33]. Here, HMGB2 is linked to tarsal-carpal coalition syndrome.