Moreover, using a more comprehensive Ago2-based RNA immunoprecipitation to immunoprecipate Ago2-RNA complexes followed by RNA sequencing (Ago2 RIP-seq) approach, we profiled the miRNomes in NPCs from ischemic rats and found that stroke substantially changed Ago2-associated miRNA profiles in NPCs compared to those in non-ischemic NPCs. This evidence concerns the gene AGO2 and Stroke.