MPE-resident CD8+ T cells were bead-isolated, rested for 24 h in complete media supplemented with 6000 IU/mL IL-2, and subsequently co-cultured for 24 h at a 1:1 ratio with either autologous tumor containing non-hematopoietic cells from the MPE or autologous peripheral blood monocytes. Here, CD8A is linked to neoplasm.