Metformin significantly reduced A4 (P = 0.036), T (P = 0.023), and SHBG (P = 0.010) circulating levels throughout pregnancy in PCOS mothers with a male fetus, while it did not have any effect on A4, T, or SHBG levels in women with PCOS with a female fetus. The gene discussed is SHBG; the disease is polycystic ovary syndrome.