Mean nicotinamide levels in the subjects without NAFLD having elevation of ALT and subjects with NAFLD having elevation of ALT of ALT were 2.860E-04 ± 1.064E-04 and 3.877E-04 ± 1.284E-04, respectively, which was a statistically significant difference (p < 0.05). This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.