The integration of the NP-based sonodynamic treatment framework of immunotherapy blockade and immune adjuvant avoids tumor metastasis and induces an antitumor and immune response by inducing robust immune responses, including increased maturation of DCs, CD4 + and CD8 + lymphocyte infiltration, CD45 + leucocytes, and cytokine secretion (Saeed et al., 2019). This evidence concerns the gene CD4 and neoplasm.