AKT1 and melanoma: Western blot analysis of the effect of HI-511 (0, 0.625, 1.25, 2.5, 5 μM) revealed a dose-dependent reduction of the phosphorylation level of histone H3 (S10), MEK, ERKs, and AKT in both vemurafenib-sensitive (A375 and M249) and -resistant melanoma cell lines (A375R and M249R; Figures 7E, F; Figures S7C, D).