To shed light on its effects during bladder cancer progression, we used two low-invasive (RT-4 and RT-112) and two high-invasive (T24 and UMUC-3) BC cell lines (Figure 2A and Figure S1A) and monitored whether overexpression or knockdown of SLC16A1-AS1 affects their invasion and proliferation capacity. The gene discussed is SLC16A1; the disease is urinary bladder cancer.