SIRT4 and cancer: To further address the role of SIRT4 in cancer stemness, we next generated MDA-MB-231, BT549, and SK-BR-3 cells overexpressing SIRT4, and MDA-MB-468, MCF-7 as well as MCF-10A cells knocking down endogenous SIRT4 via lentiviral shRNA (Figure 3G-H; Figure S6; Figure S7A-D).