In this study, RPS6 knockdown resulted in decreased Cyclin E, CDK2, Cyclin D1, CDK4, and CDK6 levels, and reduced Rb phosphorylation, thus hindering the transition from G0G1 to S phase, causing most cells to be blocked in G0G1 phase, thereby inhibiting ovarian cancer cell growth. This evidence concerns the gene RB1 and ovarian cancer.