The number of macrophages/microglia per FOV that were double immunopositive for GSDMD and cleaved caspase-3 was also significantly increased in MS lesions compared to both NAWM (p < 0.001) and nonMS brains (p < 0.0001) (Supplemental Figure 2D), and in NAWM compared to nonMS controls (p < 0.0001). Here, GSDMD is linked to myeloid sarcoma.