Using MS and EAE as prototypic models of neuroinflammation, we first verified our earlier studies that had demonstrated GSDMD immunoreactivity in Iba-1 immunopositive macrophages/microglia; although there were subtle differences in the EAE model (the disease course in the current study was more severe and tissue was harvested during the chronic rather than peak stage of disease), the overall percentage of GSDMD immunopositive macrophages/microglia in spinal cord lesions was consistent (~ 80%) in both studies (Fig. 8(C)) [9]. This evidence concerns the gene AIF1 and myeloid sarcoma.