As revealed by the Western blot analysis for Syt5 (Supplementary Fig. 5a), immunoreactivity for this protein was distinctly decreased in the whole cell protein extract from SiMa neuroblastoma cells transfected with the Syt5 shRNA expression vector, as compared with untreated cells, and also to cells transfected with a non-mammalian shRNA expression vector for control purposes. The gene discussed is SYT5; the disease is neuroblastoma.