AT8/N368-positive Tau is also ThS-positive in the LC region of 12-month-old 3×Tg mice, 6-month-old Tau P301S mice, and human AD subjects, supporting that hyperphosphorylated Tau is aggregated, and Tau fibrillization is further confirmed by Gallyas-Braak staining. This evidence concerns the gene MAPT and Alzheimer disease.